Sequence divergence of mitochondrial DNA indicates cryptic biodiversity in Octopus vulgaris and supports the taxonomic distinctiveness of Octopus mimus (Cephalopoda: Octopodidae)

DNA sequence diversity of octopods was investigated using the mitochondrial cytochrome oxidase III gene (mtCOIII). DNA was obtained from ethanol- or formalin-fixed tissue of 15 specimens belonging to Octopus mimus Gould, 1852, Octopus vulgaris Cuvier, 1797 and Scaeurgus unicirrhus d'Orbigny, 1840, from coastal waters of the Mediterranean (France), the southwestern Atlantic (Brazil), the Caribbean (Costa Rica) and the southeastern/tropical Pacific (north Chile/Costa Rica). A 612 bp fragment of the mtCOIII gene was sequenced and aligned to the orthologous sequences available from northeastern Pacific Octopus species. Possible phylogenetic relationships were reconstructed. The mtCOIII gene tree revealed two main clusters, one comprised O. rubescens, O. dofleini and O. californicus, while the other included all O. vulgaris specimens, O. bimaculatus, O. bimaculoides and O. mimus. With one exception all South American haplotypes including O. bimaculatus and O. bimaculoides appeared as the sister group of the Mediterranean haplotype of O. vulgaris, indicating that most of the South American O. vulgaris specimens investigated would not belong to the recently redescribed species O. vulgaris restricted to the Mediterranean and eastern Atlantic. The treatment of O. mimus as a species distinct from Mediterranean O. vulgaris is supported by a high nucleotide divergence of 12.7%. Based on the mtCOIII gene tree the existence of cryptic species among O. vulgaris-like octopods is suggested. Received: 15 January 1999 / Accepted: 15 October 1999     

Epiphytische Flechten im Wandel von Immissionen und Klima • Ergebnisse einer Vergleichskartierung 1989/2007 in Nordwestdeutschland

Epiphytic lichens as indicators for changes in air pollution and climate. Results of a comparative survey 1989/2007 in north-west Germany Background, aim, and scope Lichens growing on tree bark (epiphytic lichens) respond very sensitively to environmental effects such as chemical substances and air temperature. Therefore, they are used as biomonitors for atmospheric pollution in environmental assessments. Based on a survey of epiphytic lichens in 1989, a repetition was performed in an intensively-used agricultural area of north-west Germany in 2007. The objective of this study was to assess possible changes in air pollution and climate. Materials and methods The study is based on a comparative inventory of epiphytic lichens, growing on 335 trees at 45 monitoring sites. A simplified half quantitative survey technique of the first survey was used. Results Indeed, major changes to the epiphytic lichen flora were found. Overall, nearly all monitoring points showed an increase in the level of lichen species. A sharp decrease in acidophileous species and a sharp increase in basidophileous and nitrophileous species were detected. In addition, an increase in thermophileous species which are mainly inhabitants of southern European countries was observed, combined with decreases in boreo-montanic species. Discussion These trends correspond with supra-regional observations. They are primarily attributed to changes in air pollution involving a decrease in SO₂ and an increase in NH₃ concentrations. Clear effects from climate change are evident as well. Conclusions Changes to epiphytic lichens over a 18-year period could be demonstrated using a relatively low-cost investigation. They are relevant for assessing the changing environmental situation, which is of great importance for other organism groups and ecosystems. Recommendations and perspectives Using standardized techniques epiphytic lichens are suitable bioindicators for obtaining different types of information about the air pollution in urban areas and in intensively-used agricultural regions. Furthermore they are obviously good indicators of temperature changes in their environment. More research is needed about the suitability of epiphytic lichens for a biomonitoring of climate changes.     

Immobilization of pentachlorophenol in soil using carbonaceous material amendments

In this study, three pentachlorophenol (PCP) laboratory-spiked and one field-contaminated soil were amended with 2.0% char, humic acid (HA) and peat, respectively. The amended soils were aged for either 7 or 250 days. After amendment, CaCl₂ extractability of PCP was significantly decreased. Desorption kinetics indicated that the proposed amendment could lead to a strong binding and slow desorption of PCP in soils. Amendment with char reduced the bioaccumulation factor (BAF) of PCP most significantly for earthworms (Eisenia fetida) in all soils studied. The results of both physicochemical and biological tests suggested that amendment reduced PCP bioavailability quickly and enduringly, implying that carbonaceous material amendment, especially char amendment, was a potentially attractive in situ remediation method for sequestration of PCP in contaminated soil.     

Modelling the spatial distribution of ammonia emissions in the UK

Ammonia emissions (NH3) are characterised by a high spatial variability at a local scale. When modelling the spatial distribution of NH3 emissions, it is important to provide robust emission estimates, since the model output is used to assess potential environmental impacts, e.g. exceedance of critical loads. The aim of this study was to provide a new, updated spatial NH3 emission inventory for the UK for the year 2000, based on an improved modelling approach and the use of updated input datasets. The AENEID model distributes NH3 emissions from a range of agricultural activities, such as grazing and housing of livestock, storage and spreading of manures, and fertilizer application, at a 1-km grid resolution over the most suitable landcover types. The results of the emission calculation for the year 2000 are analysed and the methodology is compared with a previous spatial emission inventory for 1996.     

Mass balance assessment of triclosan removal during conventional sewage treatment

The antimicrobial agent triclosan (5-chloro-2-(2,4-dichlorophenoxy)phenol; TCS) is a member of a larger group of polychlorinated binuclear aromatic compounds frequently associated with adverse environmental and human health effects. Whereas the structure and function of TCS would suggest significant resistance to biotransformation, biological wastewater treatment currently is considered the principal destructive mechanism limiting dispersal of and environmental contamination with this compound. We explored the persistence of TCS in a typical full-scale activated sludge US sewage treatment plant using a mass balance approach in conjunction with isotope dilution liquid chromatography electrospray ionization mass spectrometry (ID-LC-ESI-MS) for accurate quantification. Average influent and effluent concentrations (mean +/- SD) of 4.7+/-1.6 and 0.07+/-0.06 microg 1(-1), respectively, revealed an apparent (liquid-phase) removal efficiency of 98+/-1%. However, further analyses demonstrated that the particle-active TCS (80+/-22% particle-associated in influent) was sequestered into wastewater residuals and accumulated in dewatered, digested sludge to concentrations of 30000+/-11000 microg kg-1. Overall, 50+/-19% (1640+/-610 g d-1) of the disinfectant mass entering the plant (3240+/-1860 g d-1) remained detectable in sludge, and less than half of the total mass (48+/-19%) was biotransformed or lost to other mechanisms. Thus, conventional sewage treatment was demonstrated to be much less effective in destroying the antimicrobial than the aqueous-phase removal efficiency of the plant would make believe. Furthermore, study findings indicate that the common practice of sludge recycling in agriculture results in the transfer of substantial quantities of TCS to US soils used, in part, for animal husbandry and crop production.     

Revealing Preferences for Reductions of Public Risks: An Application of the CV Approach

In this paper the contingent valuation method (CVM) is applied to public risk reduction. Theory suggests that the size of the stated willingness to pay (WTP) for risk reduction depends on private activities aimed at reducing the risk level for the individual. The empirical results suggest that considering private averting activities increases the explanatory power of the estimated WTP function significantly. Thus, WTP passes the scope test which proposes that larger reductions from a larger baseline risk should be valued higher than lower risk reductions from a lower baseline risk. Furthermore, we demonstrate that the follow-up response format induces anchoring effects.     

Persistent organochlorine residues in estuarine and marine sediments from Ha Long Bay, Hai Phong Bay, and Ba Lat Estuary, Vietnam

To assess the organochlorine contamination in the northeast coastal environment of Vietnam, a total of 41 surface sediments were collected from Ha Long Bay, Hai Phong Bay, and Ba Lat estuary, and analyzed for their organochlorine content. Organochlorine compounds (OCs) were widely distributed in the Vietnamese coastal environment. Among the OCs measured, DDT compounds predominated with concentrations ranging from 0.31 to 274 ng g(-1). The overall contamination level of DDTs in coastal sediments from northern Vietnam is comparable with those from other Asian countries. However, concentrations exceeding 100 ng g(-1) are comparable with high concentrations reported from India and China, the largest DDT consumers in the world. The overall concentrations of PCBs, HCHs, and chlordanes in surface sediments were in the ranges of 0.04-18.71 ng g(-1), not detected (n.d.) - 1.00 ng g(-1), and n.d. - 0.75 ng g(-1), respectively. Ha Long Bay and Hai Phong Bay were relatively more contaminated with DDTs and PCBs than other regions, respectively. In contrast, the distribution of HCHs was relatively homogeneous. OCs contamination in the coastal environment of Vietnam is closely related to shipping and industrial activities. The levels of DDT compounds in harbors and industrial areas exceeded their sediment quality guideline values suggested by Environment Canada [CCME (Canadian Council of Ministers of the Environment), 2002. Canadian sediment quality guidelines for the protection of aquatic life. In: Canadian Environmental Quality Guidelines. Canadian Council of Ministers of the Environment, Winnipeg, MB] and Australian and New Zealand [ANZECC and ARMCANZ, 2000. National water quality management strategy. Paper No. 4, Australian and New Zealand Guidelines for Fresh and Marine Water Quality, vol. 1, The Guidelines. Australia. Document: http://www.deh.gov.au/water/quality/nwqms/volume1.html], indicating that adverse effects may occur to marine species in that areas.     

Bioaccumulation and toxicity of a cationic surfactant (DODMAC) in sediment dwelling freshwater invertebrates

Dimethyldioctadecylammonium chloride (DODMAC, CAS No. 107-64-2) is the principal active component of Di(hydrogenated tallow alkyl) dimethylammonium chloride (DHTDMAC, CAS No. 61789-80-8), a cationic surfactant formerly used principally in laundry fabric softeners. After discharge to water, DODMAC partitions strongly to sediment, therefore the assessment of the effects of DODMAC to benthic organisms is essential in any risk assessment. Chronic toxicity studies were conducted with Lumbriculus variegatus (Oligochaete), Tubifex tubifex (Oligochaete) and Caenorhabditis elegans (Nematode). NOECs were greater than 5738, 1515 and 1351 mg/kg dw, respectively, even for sub-lethal effects. Measurement of the route of uptake of DODMAC by L. variegatus demonstrated the relative importance of uptake via ingestion (86%) compared with direct contact with the sediment and via pore water (14%). The overall tendency of DODMAC to bioaccumulate, however, was low with measured accumulation factors of 0.22 and 0.78 for L. variegatus and T. tubifex, respectively.